Journal: Cell Transplantation

Article Title: Modulation of Alloimmune Responses by Interleukin-10 Prevents Rejection of Implanted Allogeneic Smooth Muscle Cells and Restores Postinfarction Ventricular Function

doi: 10.3727/096368914x681036

Figure Lengend Snippet: Figure 7. IL-10 expression in SMCs was associated with induction of Treg cells. SMCs were cocultured with naive autologous and transduced or nontransduced allogeneic leukocytes, and the effect of SMCs on Treg cell induction was assessed. (A) Representative flow cytometry plots of CD4+CD25+ T-cells in the mixed leukocyte population. (B) Quantification of flow data; the percentage of CD4+CD25+ T-cells was significantly greater in the IL-10-transduced SMC group than the other groups (p < 0.001). Control is leukocyte activated with PHA, without SMCs. (C, D) CD4+ T-cells isolated from the total leukocyte population were cocultured with SMCs. CD25 expression by RT-PCR (C) and Western blot (D) was greater in the IL-10-transduced SMC group compared to the other groups. Control is T-cell activated with PHA. Data shown are representative of two independent experiments (n = 3 in each experiment).

Article Snippet: CD4+ T-lymphocytes were isolated from a single cell suspension of the total leukocyte population using the MagCellect Rat CD4+ T-Cell Isolation Kit (R&D Systems).

Techniques: Expressing, Flow Cytometry, Control, Isolation, Reverse Transcription Polymerase Chain Reaction, Western Blot